Vegetative propagation using tissue culture / puppies. Tissue culture material using lateral buds from the tree or plant stem gerberas are healthy and of a superior kind. Seedling obtained from a Gerbera plant clumps anakannya lot, its parent prolific flowering, the growth of normal, healthy and superior types derived from plants. Purposes of seedling to be planted in open fields 1 ha around 80000-90000 when the spacing of 25 x 40 cm.
Preparation of Seeds: Seeds from seed sown before dipindahtanamkan to the field. Seeding can be done in tanks or seeding pots and small pots with a diameter large enough. Seedling media should be given a plastic lid to humidity and air temperature remains stable and protected from direct sun. Seeds obtained from the tissue culture of buds taken from the kind of superior immediately inserted into a container containing a material that is Clorax 30% sterilization. Perform sterilization for 20 minutes.
After sterilization with Clorax immediately re-sterilized with 20% HgCl2 for 5 minutes, then rinse with sterile water aquades 5 X. Seeds are separated from the clump of saplings that have been cleaned Gerbera from the ground, some of the old leaf stalks and roots discarded. Each part of at least one seedling.
Seed Seeding Technique:
Seeding in the nursery tanks: Select the location where the seedlings that gets morning sun or in a room that gets 40 watt/m2 artificial light. Prepare a mixture of media seedlings fine soil, sand and manure that has been cooked in the ratio 1:1:1. Give a thin white plastic lid so that the humidity reached 98%. Before placing a layer of media seedling insert broken bricks or tiles approximately 1 / 3 tub nursery. Then fill in 90% seedling media. Seeding Gerbera seeds evenly. After 5-7 days, the lid is opened for 1 hour in the morning. From 7-10 days after seedling lid is opened for 3 hours / day, kemudain the top of the lid is opened to 20 cm from the top to get a humidity of 90%. At the age of seedlings reached 21 days, in the afternoon lifted the lid.
Seeding in tissue culture: Prepare the basic medium of Murashige Skoog medium plus sugar 30 grams / liter, vitamin B and plant growth regulators kinetin plus IAA 5 mg 0.5 mg / liter. PH before heated arranged around pH 5.7 by adding NaOH or HCl 0.1 N Made of solid medium with Difco Bacto Agar (DBA) as much as 7.5 grams / liter. Plant the lateral buds, at the age of 45 days buds begin to form a compound. Results of tissue culture seedlings transferred to nursery and kept sterile until quite large. The next regular nursery seedlings moving into the same medium with the composition of the seed nurseries.
Seeding in the nursery tanks: Select the location where the seedlings that gets morning sun or in a room that gets 40 watt/m2 artificial light. Prepare a mixture of media seedlings fine soil, sand and manure that has been cooked in the ratio 1:1:1. Give a thin white plastic lid so that the humidity reached 98%. Before placing a layer of media seedling insert broken bricks or tiles approximately 1 / 3 tub nursery. Then fill in 90% seedling media. Seeding Gerbera seeds evenly. After 5-7 days, the lid is opened for 1 hour in the morning. From 7-10 days after seedling lid is opened for 3 hours / day, kemudain the top of the lid is opened to 20 cm from the top to get a humidity of 90%. At the age of seedlings reached 21 days, in the afternoon lifted the lid.
Seeding in tissue culture: Prepare the basic medium of Murashige Skoog medium plus sugar 30 grams / liter, vitamin B and plant growth regulators kinetin plus IAA 5 mg 0.5 mg / liter. PH before heated arranged around pH 5.7 by adding NaOH or HCl 0.1 N Made of solid medium with Difco Bacto Agar (DBA) as much as 7.5 grams / liter. Plant the lateral buds, at the age of 45 days buds begin to form a compound. Results of tissue culture seedlings transferred to nursery and kept sterile until quite large. The next regular nursery seedlings moving into the same medium with the composition of the seed nurseries.
Seeding with saplings: Plant seedlings or seeds that have been cleaned of soil, the roots of old leaves also invested in land nursery with a distance of 5 x 10 Cm. Maintenance nurseries / nursery: Flush every day 1 or 2 times depending on the weather. Fertilization is done 3 weeks after seedling. Fertilizer solution consisting of 50-10 grams of NPK in a solution of 10 liters of water, while the leaf fertilizer concentration is adjusted with the recommendation. Spacing after the age of 5-6 weeks.
Displacement Seeds: Seeds are ready moved derived seed after 3-5 strands of leafy plants. Seedlings derived from tissue culture ready for planting when the size is quite large, while the seeds are planted from seedlings ready to be moved after the seedlings are strong enough.
Displacement Seeds: Seeds are ready moved derived seed after 3-5 strands of leafy plants. Seedlings derived from tissue culture ready for planting when the size is quite large, while the seeds are planted from seedlings ready to be moved after the seedlings are strong enough.
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